Are Internal Fragments Observable in Electron Based Top-Down Mass Spectrometry?

ABSTRACT: Protein tandem mass spectrometry (MS/MS) often generates sequence-informative fragments from backbone bond cleavages near the termini. This lack of fragmentation in the protein interior is particularly apparent in native top-down mass spectrometry (MS). Improved sequence coverage, critical for reliable annotation of posttranslational modifications and sequence variants, may be obtained from internal fragments generated by multiple backbone cleavage events. However, internal fragment assignments can be error prone due to isomeric/isobaric fragments from different parts of a protein sequence. Also, internal fragment generation propensity depends on the chosen MS/MS activation strategy. Here, we examine internal fragment formation in electron capture dissociation (ECD) and electron transfer d

SUBMITTER: Mikawy NN 

PROVIDER: S-EPMC11388692 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature