Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.

ABSTRACT: Unintended gene flow from transgenic plants via pollen, seed and vegetative propagation is a regulatory concern because of potential admixture in food and crop systems, as well as hybridization and introgression to wild and weedy relatives. Bioconfinement of transgenic pollen would help address some of these concerns and enable transgenic plant production for several crops where gene flow is an issue. Here, we demonstrate the expression of the restriction endonuclease EcoRI under the control of the tomato pollen-specific LAT52 promoter is an effective method for generating selective male sterility in Nicotiana tabacum (tobacco). Of nine transgenic events recovered, four events had very high bioconfinement with tightly controlled EcoRI expression in pollen and negligible-to-no expression ot

SUBMITTER: Millwood RJ 

PROVIDER: S-EPMC11389094 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature