Transcript profiling of jasmonate-elicited Taxus cells reveals a β-phenylalanine-CoA ligase.

ABSTRACT: Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer compounds, several aspects of their biosynthesis remain unknown. In this work, a genomewide expression analysis of jasmonate-elicited Taxus baccata cell cultures by complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) indicated a correlation between an extensive elicitor-induced genetic reprogramming and increased taxane production in the targeted cultures. Subsequent in silico analysis allowed us to identify 15 genes with a jasmonate-induced differential expres

SUBMITTER: Ramirez-Estrada K 

PROVIDER: S-EPMC11389183 | biostudies-literature | 2016 Jan

REPOSITORIES: biostudies-literature