The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis.

ABSTRACT:

Background

N6-Methyladenosine (m⁶A) methylation, a common form of RNA modification, play an important role in the pathogenesis of various diseases and in the ontogeny of organisms. Nevertheless, the precise function of m⁶A methylation in photoaging remains unknown.

Objectives

This study aims to investigate the biological role and underlying mechanism of m⁶A methylation in photoaging.

Methods

m⁶A dot blot, Real-time quantitative PCR (RT-qPCR), western blot and immunohistochemical (IHC) assays were employed to detect the m⁶A level and specific m⁶A methylase in ultraviolet ray (UVR)-induced photoaging tissue. The profile of m⁶A-tagged mRNA was identified by methylated RNA immunoprecipitation seq

SUBMITTER: Liu Y 

PROVIDER: S-EPMC11389345 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature