Bivalent chromatin accommodates survivin and BRG1/SWI complex to activate DNA damage response in CD4⁺ cells.

ABSTRACT:

Background

Bivalent regions of chromatin (BvCR) are characterized by trimethylated lysine 4 (H3K4me3) and lysine 27 on histone H3 (H3K27me3) deposition which aid gene expression control during cell differentiation. The role of BvCR in post-transcriptional DNA damage response remains unidentified. Oncoprotein survivin binds chromatin and mediates IFNγ effects in CD4⁺ cells. In this study, we explored the role of BvCR in DNA damage response of autoimmune CD4⁺ cells in rheumatoid arthritis (RA).

Methods

We performed deep sequencing of the chromatin bound to survivin, H3K4me3, H3K27me3, and H3K27ac, in human CD4⁺ cells and identified BvCR, which possessed all three histone H3 modifications. Protein partners of survivin on chromatin were predicted

SUBMITTER: Chandrasekaran V 

PROVIDER: S-EPMC11389452 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature