Human intermediate prostate cancer stem cells contribute to the initiation and development of prostate adenocarcinoma.

ABSTRACT:

Background

Intermediate cells are present in the early stages of human prostate development and adenocarcinoma. While primary cells isolated from benign human prostate tissues or tumors exhibit an intermediate phenotype in vitro, they cannot form tumors in vivo unless genetically modified. It is unclear about the stem cell properties and tumorigenicity of intermediate cells.

Methods

We developed a customized medium to culture primary human intermediate prostate cells, which were transplanted into male immunodeficient NCG mice to examine tumorigenicity in vivo. We treated the cells with different concentrations of dihydrotestosterone (DHT) and enzalutamide in vitro and surgically castrated the mice after cell transplantation in vivo. Immunostaining, qRT-PCR, RNA sequencing, a

SUBMITTER: Mu J 

PROVIDER: S-EPMC11389492 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature