Designing Michaelases: exploration of novel protein scaffolds for iminium biocatalysis.

ABSTRACT: Biocatalysis is becoming a powerful and sustainable alternative for asymmetric catalysis. However, enzymes are often restricted to metabolic and less complex reactivities. This can be addressed by protein engineering, such as incorporating new-to-nature functional groups into proteins through the so-called expansion of the genetic code to produce artificial enzymes. Selecting a suitable protein scaffold is a challenging task that plays a key role in designing artificial enzymes. In this work, we explored different protein scaffolds for an abiological model of iminium-ion catalysis, Michael addition of nitromethane into E-cinnamaldehyde. We studied scaffolds looking for open hydrophobic pockets and enzymes with described binding sites for the targeted substrate. The proteins were exp

SUBMITTER: Gran-Scheuch A 

PROVIDER: S-EPMC11389850 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature