Development and validation of next-generation sequencing panel for personalized Helicobacter pylori eradication treatment targeting multiple species.

ABSTRACT:

Introduction

The decreasing Helicobacter pylori eradication rate is primarily attributed to antibiotic resistance, and further exacerbated by uniform drug administration disregarding a host's metabolic capability. Consequently, applying personalized treatment based on antibiotic resistance-associated variants and the host's metabolic phenotype can potentially increase the eradication rate.

Method

A custom next-generation sequencing panel for personalized H. pylori eradication treatment (NGS-PHET) was designed which targeted the regions for amoxicillin, clarithromycin, metronidazole, tetracycline, and levofloxacin-resistance in H. pylori and human proton-pump inhibitor (PPI) metabolism. The libraries were constructed following customized methods and seque

SUBMITTER: Min BJ 

PROVIDER: S-EPMC11390507 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature