Functional mouse hepatocytes derived from interspecies chimeric livers effectively mitigate chronic liver fibrosis.

ABSTRACT: Liver disease is a major global health challenge. There is a shortage of liver donors worldwide, and hepatocyte transplantation (HT) may be an effective treatment to overcome this problem. However, the present approaches for generation of hepatocytes are associated with challenges, and interspecies chimera-derived hepatocytes produced by interspecies blastocyst complementation (IBC) may be promising donor hepatocytes because of their more comprehensive hepatic functions. In this study, we isolated mouse hepatocytes from mouse-rat chimeric livers using IBC and found that interspecies chimera-derived hepatocytes exhibited mature hepatic functions in terms of lipid accumulation, glycogen storage, and urea synthesis. Meanwhile, they were more similar to endogenous hepatocytes than hepatocytes

SUBMITTER: Huang C 

PROVIDER: S-EPMC11390683 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature