Modeling cancer-associated hypercoagulability using glioblastoma spheroids in microfluidic chips.

ABSTRACT:

Background

Cancer increases the risk of venous thromboembolism, and glioblastoma is one of the cancer types with the highest risk of venous thromboembolism (10%-30%). Tumor-intrinsic features are believed to affect vascular permeability and hypercoagulability, but novel models are required to study the pathophysiological dynamics underlying cancer-associated thrombosis at the molecular level.

Objectives

We have developed a novel cancer-on-a-chip model to examine the effects of glioblastoma cells on the deregulation of blood coagulation.

Methods

This was accomplished by coculturing vessel-forming human umbilical vein endothelial cells with glioblastoma spheroids overexpressing tissue factor (TF), the initiator of coagulation (U251 lentivirus, LV-TF) or an LV-control (U

SUBMITTER: Kapteijn MY 

PROVIDER: S-EPMC11391032 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature