Exploring the molecular mechanisms of macrophages in islet transplantation using single-cell analysis.

ABSTRACT:

Background

Islet transplantation is a promising treatment for type 1 diabetes that aims to restore insulin production and improve glucose control, but long-term graft survival remains a challenge due to immune rejection.

Methods

ScRNA-seq data from syngeneic and allogeneic islet transplantation grafts were obtained from GSE198865. Seurat was used for filtering and clustering, and UMAP was used for dimension reduction. Differentially expressed genes were analyzed between syngeneic and allogeneic islet transplantation grafts. Gene set variation analysis (GSVA) was performed on the HALLMARK gene sets from MSigDB. Monocle 2 was used to reconstruct differentiation trajectories, and cytokine signature enrichment analysis was used to compare cytokine responses between syngeneic and

SUBMITTER: Pu Z 

PROVIDER: S-EPMC11391485 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature