Identification of macrophage driver genes in fibrosis caused by different heart diseases based on omics integration.

ABSTRACT:

Background

Myocardial fibrosis, a hallmark of heart disease, is closely associated with macrophages, yet the genetic pathophysiology remains incompletely understood. In this study, we utilized integrated single-cell transcriptomics and bulk RNA-seq analysis to investigate the relationship between macrophages and myocardial fibrosis across omics integration.

Methods

We examined and curated existing single-cell data from dilated cardiomyopathy (DCM), ischemic cardiomyopathy (ICM), myocardial infarction (MI), and heart failure (HF), and analyzed the integrated data using cell communication, transcription factor identification, high dimensional weighted gene co-expression network analysis (hdWGCNA), and functional enrichment to elucidate the drivers of macrophage polarization an

SUBMITTER: Zhang YZ 

PROVIDER: S-EPMC11391649 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature