TRPM4 inhibition slows neuritogenesis progression of cortical neurons.

ABSTRACT: TRPM4 is a non-selective cation channel activated by intracellular Ca²⁺ but only permeable to monovalent cations, its activation regulates membrane potential and intracellular calcium. This channel participates in the migration and adhesion of non-excitable cells and forms an integral part of the focal adhesion complex. In neurons, TRPM4 expression starts before birth and its function at this stage is not clear, but it may function in processes such as neurite development. Here we investigate the role of TRPM4 in neuritogenesis. We found that neurons at DIV 0 express TRPM4, the inhibition of TRPM4 using 9-Ph reduces neurite number and slows the progression of neurite development, keeping neurons in stage 1. The genetic suppression of TRPM4 using an shRNA at later stages (DIV2) r

SUBMITTER: Riquelme D 

PROVIDER: S-EPMC11391768 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature