Synthesis of propargyl glycosides of <i>Streptococcus pneumoniae</i> serotypes 6A and 6B for glycoconjugate vaccines.

ABSTRACT: We developed a method for making immune responses to bacterial glycans T cell-dependent, which involves attachment of short, synthetic glycans to a virus-like nanoparticle (VLP). This strategy enhances immune responses to glycans by facilitating cognate T cell help of B cells, leading to antibody class switching and affinity maturation yielding high-affinity, anti-glycan antibodies. This method requires synthesis of bacterial glycans as propargyl glycosides for covalent attachment to VLPs, and the resulting short linker between the VLP and glycan is important for optimal T cell receptor recognition. In this work, glycans that are part of the capsular polysaccharides (CPS) produced by Streptococcus pneumoniae serotypes Sp6A and Sp6B were synthesized as disaccharides and trisaccharide

SUBMITTER: Meng T 

PROVIDER: S-EPMC11391900 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature