Automated Ligand Purification Platform Accelerates Immunopeptidome Analysis by Mass Spectrometry.

ABSTRACT: Major histocompatibility complex (MHC)-presented peptides (pMHC) give insight into T cell immune responses, a critical step toward developing a new generation of targeted immunotherapies. Recent instrumentation advances have propelled mass spectrometry to being arguably the most robust technology for discovering and quantifying naturally presented pMHC from cells and tissues. However, sample preparation has remained a major limitation due to time-consuming and labor-intensive workflows. We developed a high-throughput and automated platform with enhanced speed, sensitivity, and reproducibility relative to prior studies. This pipeline is capable of processing up to 96 samples in 6 h or less yielding high-quality pMHC mixtures ready for mass spectrometry. Here, we describe our efforts to opti

SUBMITTER: Zhang L 

PROVIDER: S-EPMC11391901 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature