Restoring Atrial T-Tubules Augments Systolic Ca Upon Recovery From Heart Failure.

ABSTRACT:

Background

Transverse (t)-tubules drive the rapid and synchronous Ca²⁺ rise in cardiac myocytes. The virtual complete atrial t-tubule loss in heart failure (HF) decreases Ca²⁺ release. It is unknown if or how atrial t-tubules can be restored and how this affects systolic Ca²⁺.

Methods

HF was induced in sheep by rapid ventricular pacing and recovered following termination of rapid pacing. Serial block-face scanning electron microscopy and confocal imaging were used to study t-tubule ultrastructure. Function was assessed using patch clamp, Ca²⁺, and confocal imaging. Candidate proteins involved in atrial t-tubule recovery were identified by western blot and expressed in rat neonatal ventricular myocytes to determine if they altered t

SUBMITTER: Caldwell JL 

PROVIDER: S-EPMC11392124 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature