A monomeric StayGold fluorescent protein.

ABSTRACT: StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.

SUBMITTER: Ivorra-Molla E 

PROVIDER: S-EPMC11392804 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature