High-quality metagenome assembly from long accurate reads with metaMDBG.

ABSTRACT: We introduce metaMDBG, a metagenomics assembler for PacBio HiFi reads. MetaMDBG combines a de Bruijn graph assembly in a minimizer space with an iterative assembly over sequences of minimizers to address variations in genome coverage depth and an abundance-based filtering strategy to simplify strain complexity. For complex communities, we obtained up to twice as many high-quality circularized prokaryotic metagenome-assembled genomes as existing methods and had better recovery of viruses and plasmids.

SUBMITTER: Benoit G 

PROVIDER: S-EPMC11392814 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature