Oncostatin M receptor-dependent signaling assessed by RNA sequencing in mouse hematopoietic stem cells.

ABSTRACT: Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family of cytokines and has been found to have anti-inflammatory and pro-inflammatory properties in various cellular and disease contexts. OSM signals through two receptor complexes, one of which includes OSMRβ. Here, we investigated OSM-OSMRβ signaling in adult mouse hematopoietic stem cells (HSCs) using the conditional Osmr^fl/fl mouse model B6;129-Osmr^tm1.1Nat/J. We crossed Osmr^fl/fl mice to interferon-inducible Mx1-Cre, which is robustly induced in adult HSCs. From these mice, we isolated HSCs by flow cytometry, stimulated with recombinant OSM or vehicle for 1 hour, and assessed gene expression changes in control versus Osmr knockout HSCs by RNA-seq. This data may be utilized to investigate OSMRβ

SUBMITTER: Schwartz LS 

PROVIDER: S-EPMC11392925 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature