Phenotypic profiling of human induced regulatory T cells at early differentiation: insights into distinct immunosuppressive potential.

ABSTRACT: Regulatory T cells (Tregs) play a key role in suppressing systemic effector immune responses, thereby preventing autoimmune diseases but also potentially contributing to tumor progression. Thus, there is great interest in clinically manipulating Tregs, but the precise mechanisms governing in vitro-induced Treg (iTreg) differentiation are not yet fully understood. Here, we used multiparametric mass cytometry to phenotypically profile human iTregs during the early stages of in vitro differentiation at single-cell level. A panel of 25 metal-conjugated antibodies specific to markers associated with human Tregs was used to characterize these immunomodulatory cells. We found that iTregs highly express the transcription factor FOXP3, as well as characteristic Treg-associated surface markers (e.g.

SUBMITTER: Kattelus R 

PROVIDER: S-EPMC11393232 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature