Characterization of a Nicotiana tabacum phytochelatin synthase 1 and its response to cadmium stress.

ABSTRACT: Phytochelatin synthase (PCS) is a critical enzyme involved in heavy metal detoxification in organisms. In this study, we aim to comprehensively investigate the molecular and functional characteristics of the PCS1 gene from Nicotiana tabacum by examining its enzymatic activity, tissue-specific expression pattern, Cd-induced expression, as well as the impact on Cd tolerance and accumulation. The results demonstrated that the amino acid sequence of NtPCS1 shared a high similarity in its N-terminal region with PCS from other species. The enzymatic activity of NtPCS1 was found to be enhanced in the order Ag²⁺ > Cd²⁺ > Cu²⁺ > Pb²⁺ > Hg²⁺ > Fe²⁺ > Zn²⁺. In addition, RT-PCR data indicated that NtPCS1

SUBMITTER: Wu C 

PROVIDER: S-EPMC11393743 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature