Generation, characterization, and use of EKLF(Klf1)/CRE knock-in mice for cell-restricted analyses.

ABSTRACT:

Introduction

EKLF/Klf1 is a tissue-restricted transcription factor that plays a critical role in all aspects of erythropoiesis. Of particular note is its tissue-restricted pattern of expression, a property that could prove useful for expression control of a linked marker or enzymatic gene.

Methods and results

With this in mind, we fused the CRE recombinase to the genomic EKLF coding region and established mouse lines. We find by FACS analyses that CRE expression driven by the EKLF transcription unit recapitulates erythroid-restricted expression with high penetrance in developing embryos. We then used this line to test its properties in the adult, where we found EKLF/CRE is an active and is a robust mimic of normal EKLF expression in the adult bone marrow. EKLF/CRE is also ex

SUBMITTER: Xue L 

PROVIDER: S-EPMC11393758 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature