Stallion Sperm Freezing with Different Extenders: Role of Antioxidant Activity and Nitric Oxide Production.

ABSTRACT: Sensitivity to freezing remains a critical issue in stallion semen cryopreservation procedures. To explore this topic in-depth, semen was collected from ten stallions, diluted with three different extenders, transported to the laboratory, and then centrifuged and frozen with four different extenders. We conducted analyses of sperm kinetics, mitochondrial membrane potential (MMP), and hydrogen peroxide content both before and after freezing. Additionally, we assessed antioxidant activity using the ABTS and FRAP methods and measured nitric oxide stable metabolites (NOx) in the blank extenders, seminal plasma, and extenders conditioned by spermatozoa before and after freezing. We found significant variability in the antioxidant activity and NOx content of the blank extenders and the seminal p

SUBMITTER: Boni R 

PROVIDER: S-EPMC11394550 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature