Metalloproteomics Reveals Multi-Level Stress Response in Escherichia coli When Exposed to Arsenite.

ABSTRACT: The arsRBC operon encodes a three-protein arsenic resistance system. ArsR regulates the transcription of the operon, while ArsB and ArsC are involved in exporting trivalent arsenic and reducing pentavalent arsenic, respectively. Previous research into Agrobacterium tumefaciens 5A has demonstrated that ArsR has regulatory control over a wide range of metal-related proteins and metabolic pathways. We hypothesized that ArsR has broad regulatory control in other Gram-negative bacteria and set out to test this. Here, we use differential proteomics to investigate changes caused by the presence of the arsR gene in human microbiome-relevant Escherichia coli during arsenite (As^III) exposure. We show that ArsR has broad-ranging impacts such as the expression of

SUBMITTER: Larson J 

PROVIDER: S-EPMC11394912 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature