Crucial Parameters for Immunopeptidome Characterization: A Systematic Evaluation.

ABSTRACT: Immunopeptidomics is the area of knowledge focused on the study of peptides assembled in the major histocompatibility complex (MHC), or human leukocyte antigen (HLA) in humans, which could activate the immune response via specific and selective T cell recognition. Advances in high-sensitivity mass spectrometry have enabled the detailed identification and quantification of the immunopeptidome, significantly impacting fields like oncology, infections, and autoimmune diseases. Current immunopeptidomics approaches primarily focus on workflows to identify immunopeptides from HLA molecules, requiring the isolation of the HLA from relevant cells or tissues. Common critical steps in these workflows, such as cell lysis, HLA immunoenrichment, and peptide isolation, significantly influence outcomes.

SUBMITTER: Juanes-Velasco P 

PROVIDER: S-EPMC11395153 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature