Comparison of targeted next-generation sequencing and metagenomic next-generation sequencing in the identification of pathogens in pneumonia after congenital heart surgery: a comparative diagnostic accuracy study.

ABSTRACT:

Background

This study aimed to compare targeted next-generation sequencing (tNGS) with metagenomic next-generation sequencing (mNGS) for pathogen detection in infants with severe postoperative pneumonia after congenital heart surgery.

Methods

We conducted a retrospective observational study using data from the electronic medical record system of infants who developed severe pneumonia after surgery for congenital heart disease from August 2021 to August 2022. Infants were divided into tNGS and mNGS groups based on the pathogen detection methods. The primary outcome was the efficiency of pathogen detection, and the secondary outcomes were the timeliness and cost of each method.

Results

In the study, 91 infants were included, with tNGS detecting pathogens in 84.6% (77/91

SUBMITTER: Zheng YR 

PROVIDER: S-EPMC11395185 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature