Exosomal transcript cargo and functional correlation with HNSCC patients' survival.

ABSTRACT:

Background

HPV status in a subset of HNSCC is linked with distinct treatment outcomes. Present investigation aims to elucidate the distinct clinicopathological features of HPV-positive and HPV-negative HNSCC and investigate their association with the HNSCC patient survival.

Materials and methods

The total RNA of exosomes from HPV-positive (93VU147T) and HPV-negative (OCT-1) HNSCC cells was isolated, and the transcripts were estimated using Illumina HiSeq X. The expression of altered transcripts and their clinical relevance were further analyzed using publicly available cancer transcriptome data from The Cancer Genome Atlas (TCGA).

Results

Transcriptomic analyses identified 3785 differentially exported transcripts (DETs) in HPV-positive exosomes compared to HPV-negativ

SUBMITTER: Yadav J 

PROVIDER: S-EPMC11395213 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature