Single-cell sequencing of the vermiform appendix during development identifies transcriptional relationships with appendicitis in preschool children.

ABSTRACT:

Background

The development of the human vermiform appendix at the cellular level, as well as its function, is not well understood. Appendicitis in preschool children, although uncommon, is associated with a high perforation rate and increased morbidity.

Methods

We performed single-cell RNA sequencing (scRNA-seq) on the human appendix during fetal and pediatric stages as well as preschool-age inflammatory appendices. Transcriptional features of each cell compartment were discussed in the developing appendix. Cellular interactions and differentiation trajectories were also investigated. We compared scRNA-seq profiles from preschool appendicitis to those of matched healthy controls to reveal disease-associated changes. Bulk transcriptomic data, immunohistochemistry, and real-ti

SUBMITTER: Meng L 

PROVIDER: S-EPMC11395239 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature