An Inducible Luminescent System to Explore Parkinson's Disease-Associated Genes.

ABSTRACT: With emerging genetic association studies, new genes and pathways are revealed as causative factors in the development of Parkinson's disease (PD). However, many of these PD genes are poorly characterized in terms of their function, subcellular localization, and interaction with other components in cellular pathways. This represents a major obstacle towards a better understanding of the molecular causes of PD, with deeper molecular studies often hindered by a lack of high-quality, validated antibodies for detecting the corresponding proteins of interest. In this study, we leveraged the nanoluciferase-derived LgBiT-HiBiT system by generating a cohort of tagged PD genes in both induced pluripotent stem cells (iPSCs) and iPSC-derived neuronal cells. To promote luminescence signals within cell

SUBMITTER: Gandy A 

PROVIDER: S-EPMC11395715 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature