Isotope-dilution-LC-MS/MS candidate reference measurement procedure for cefepime in human serum.

ABSTRACT:

Background

Reference measurement procedures are an essential element in the standardization and comparability of analytical measurement results in laboratory medicine. No LC-MS/MS-based reference measurement procedure for cefepime in serum has been published previously.

Materials and methods

An isotope-dilution based two-dimensional LC-MS/MS reference measurement procedure for cefepime concentrations in human serum was developed and tested. The value assignment of unknown samples is based on a defined measurement series validation. Six unknown samples can be measured per series. Pass criteria for the run and the samples were determined empirically based on a performance evaluation. For this purpose, a between-run determination of five runs of the defined measurement series w

SUBMITTER: Schaffler J 

PROVIDER: S-EPMC11396040 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature