Light Emission from Fe²⁺-EGTA-H₂O₂ System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body.

ABSTRACT: A Fe²⁺-EGTA(ethylene glycol-bis (β-aminoethyl ether)-N,N,N',N'-tetraacetic acid)-H₂O₂ system emits photons, and quenching this chemiluminescence can be used for determination of anti-hydroxyl radical (•OH) activity of various compounds. The generation of •OH and light emission due to oxidative damage to EGTA may depend on the buffer and pH of the reaction milieu. In this study, we evaluated the effect of pH from 6.0 to 7.4 (that may occur in human cells) stabilized with 10 mM phosphate buffer (main intracellular buffer) on a chemiluminescence signal and the ratio of this signal to noise (light emission from medium alone). The highest signal (4698 ± 583 RLU) and signal-to-noise ratio (9.7 ± 1.5) were noted for pH 6.6. Lower and hi

SUBMITTER: Sasak K 

PROVIDER: S-EPMC11396423 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature