C-Myc alone is enough to reprogram fibroblasts into functional macrophages.

ABSTRACT:

Background

Macrophage-based cell therapy is promising in solid tumors, but the efficient acquisition of macrophages remains a challenge. Induced pluripotent stem cell (iPSC)-induced macrophages are a valuable source, but time-consuming and costly. The application of reprogramming technologies allows for the generation of macrophages from somatic cells, thereby facilitating the advancement of cell-based therapies for numerous malignant diseases.

Methods

The composition of CD45⁺ myeloid-like cell complex (MCC) and induced macrophage (iMac) were analyzed by flow cytometry and single-cell RNA sequencing. The engraftment capacity of CD45⁺ MCC was evaluated by two transplantation assays. Regulation of c-Myc on MafB was evaluated by ChIP-qPCR and promoter repo

SUBMITTER: Li S 

PROVIDER: S-EPMC11396436 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature