Expandable hESC-derived cardiovascular progenitor cells generate functional cardiac lineage cells for microtissue construction.

ABSTRACT:

Background

Cardiovascular progenitor cells (CPCs) derived from human embryonic stem cells (hESCs) are considered valuable cell sources for investigating cardiovascular physiology in vitro. Meeting the diverse needs of this application requires the large-scale production of CPCs in an in vitro environment. This study aimed to use an effective culture system utilizing signaling factors for the large-scale expansion of hESC-derived CPCs with the potential to differentiate into functional cardiac lineage cells.

Methods and results

Initially, CPCs were generated from hESCs using a 4-day differentiation protocol with a combination of four small molecules (CHIR99021, IWP2, SB-431542, and purmorphamine). These CPCs were then expanded and maintained in a medium containing three facto

SUBMITTER: Rezaeiani S 

PROVIDER: S-EPMC11396807 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature