The Effect of the Lysine Acetylation Modification of ClpP on the Virulence of Vibrio alginolyticus.

ABSTRACT: Acetylation modification has become one of the most popular topics in protein post-translational modification (PTM) research and plays an important role in bacterial virulence. A previous study indicated that the virulence-associated caseinolytic protease proteolytic subunit (ClpP) is acetylated at the K165 site in Vibrio alginolyticus strain HY9901, but its regulation regarding the virulence of V. alginolyticus is still unknown. We further confirmed that ClpP undergoes lysine acetylation (Kace) modification by immunoprecipitation and Western blot analysis and constructed the complementation strain (C-clpP) and site-directed mutagenesis strains including K165Q and K165R. The K165R strain significantly increased biofilm formation at 36 h of incubation, and K165Q signifi

SUBMITTER: Wang S 

PROVIDER: S-EPMC11396845 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature