Selection and Validation of Reference Genes in Dendrocalamus brandisii for Quantitative Real-Time PCR.
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ABSTRACT: Dendrocalamus brandisii (Munro) Kurz is a sympodial bamboo species with a wide distribution in tropical and subtropical regions. Due to its remarkable regenerative ability and exceptional flavor, this species plays a pivotal role in bolstering the economies of numerous nations across these regions. We recently published a high-quality genome of this species. To date, no study results have identified the optimal reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) normalization in Dendrocalamus brandisii. qRT-PCR offers a highly accurate and effective approach to analyzing gene expression. However, the precision of the resulting quantitative data hinges on the correct choice of reference genes. Twenty-one potential reference genes were identified from
SUBMITTER: Jiang J
PROVIDER: S-EPMC11396877 | biostudies-literature | 2024 Aug
REPOSITORIES: biostudies-literature
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