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Generic Workflow of a Highly Effective and Easy Anther Culture Method for Both Japonica and Indica Rice.


ABSTRACT: As one of the most important staple crops in the world, rice plays a pivotal role in world food security. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice. In this study, fifteen rice genotypes, including twelve japonica rice genotypes and three indica rice genotypes, were randomly selected and used to study anther culture by using a modified M8 medium. The results showed that the total callus induction rates of these different rice genotypes ranged from 0.81 to 13.95%, with an average of 6.64%, while the callus induction rates calculated for the top ten highest callus inductions for each rice g

SUBMITTER: Guo G 

PROVIDER: S-EPMC11397046 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature