Escherichia coli alcohol dehydrogenase YahK is a protein that binds both iron and zinc.

ABSTRACT:

Background

Previous studies have highlighted the catalytic activity of Escherichia coli alcohol dehydrogenase YahK in the presence of coenzyme nicotinamide adenine dinucleotide (NAD) and metal zinc. Notably, competitive interaction between iron and zinc ligands has been shown to influence the catalytic efficiency of several key proteases. This study aims to unravel the intricate mechanisms underlying YahK's catalytic action, with a particular focus on the pivotal roles played by metal ions zinc and iron.

Methods

The purified YahK protein from E. coli cells cultivated in LB medium was utilized to investigate its metal-binding properties through UV-visible absorption measurements and determination of metal content. Subsequently, the effects of excess zinc and iro

SUBMITTER: Liang F 

PROVIDER: S-EPMC11397123 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature