An Efficient Direct Conversion Strategy to Generate Functional Astrocytes from Human Adult Fibroblasts.

ABSTRACT: Direct reprogramming approaches offer an attractive alternative to stem-cell-derived models, allowing the retention of epigenetic information and age-associated cellular phenotypes, and providing an expedited method to generate target cell types. Several groups have previously generated multiple neuronal subtypes, neural progenitor cells, oligodendrocytes, and other cell types directly from fibroblasts. However, while some groups have had success at the efficient conversion of embryonic fibroblasts to astrocytes, they have not yet achieved similar conversion efficiency for adult human fibroblasts. To generate astrocytes for the study of adult-stage disorders, we developed an improved direct conversion strategy employing a combination of small molecules to activate specific pa

SUBMITTER: Bhaskar U 

PROVIDER: S-EPMC11398335 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature