Engineered Transcription Factor Binding Arrays for DNA-based Gene Expression Control in Mammalian Cells.

ABSTRACT: Manipulating gene expression in mammalian cells is critical for cell engineering applications. Here we explore the potential of transcription factor (TF) recognition element arrays as DNA tools for modifying free TF levels in cells and thereby controlling gene expression. We first demonstrate proof-of-concept, showing that Tet TF-binding recognition element (RE) arrays of different lengths can tune gene expression and alter gene circuit performance in a predictable manner. We then open-up the approach to interface with any TF with a known binding site by developing a new method called Cloning Troublesome Repeats in Loops (CTRL) that can assemble plasmids with up to 256 repeats of any RE sequence. Transfection of RE array plasmids assembled by CTRL into mammalian cells show potential to mod

SUBMITTER: Zouein A 

PROVIDER: S-EPMC11398425 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature