In silico analyses identify sequence contamination thresholds for Nanopore-generated SARS-CoV-2 sequences.

ABSTRACT: The SARS-CoV-2 pandemic has brought molecular biology and genomic sequencing into the public consciousness and lexicon. With an emphasis on rapid turnaround, genomic data informed both diagnostic and surveillance decisions for the current pandemic at a previously unheard-of scale. The surge in the submission of genomic data to publicly available databases proved essential as comparing different genome sequences offers a wealth of knowledge, including phylogenetic links, modes of transmission, rates of evolution, and the impact of mutations on infection and disease severity. However, the scale of the pandemic has meant that sequencing runs are rarely repeated due to limited sample material and/or the availability of sequencing resources, resulting in the upload of some imperfect runs to pub

SUBMITTER: Bolaji AJ 

PROVIDER: S-EPMC11398645 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature