Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor.

ABSTRACT: The formamidase (FMD) enzyme plays an important role in fungal thriving by releasing a secondary nitrogen source as a product of its activity. In Paracoccidioides species, previous studies have demonstrated the upregulation of this enzyme in a wide range of starvation and infective-like conditions. However, Paracoccidioides lutzii formamidase has not yet been defined as a virulence factor. Here, by employing in vivo infections using an fmd-silenced strain in Galleria mellonella larvae model, we demonstrate the influence of formamidase in P. lutzii's immune stimulation and pathogenicity. The formamidase silencing resulted in improper arrangement of the nodules, poor melanogenesis and decreased fungal burden. Thus, we suggest that formamidase may be a piece composing the process of molecular

SUBMITTER: Pereira ED 

PROVIDER: S-EPMC11398694 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature