Analyzing single-cell bisulfite sequencing data with MethSCAn.

ABSTRACT: Single-cell bisulfite sequencing (scBS) is a technique that enables the assessment of DNA methylation at single-base pair and single-cell resolution. The analysis of large datasets obtained from scBS requires preprocessing to reduce the data size, improve the signal-to-noise ratio and provide interpretability. Typically, this is achieved by dividing the genome into large tiles and averaging the methylation signals within each tile. Here we demonstrate that this coarse-graining approach can lead to signal dilution. We propose improved strategies to identify more informative regions for methylation quantification and a more accurate quantitation method than simple averaging. Our approach enables better discrimination of cell types and other features of interest and reduces the need for large

SUBMITTER: Kremer LPM 

PROVIDER: S-EPMC11399085 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature