Single-cell EpiChem jointly measures drug-chromatin binding and multimodal epigenome.

ABSTRACT: Studies of molecular and cellular functions of small-molecule inhibitors in cancer treatment, eliciting effects by targeting genome and epigenome associated proteins, requires measurement of drug-target engagement in single-cell resolution. Here we present EpiChem for in situ single-cell joint mapping of small molecules and multimodal epigenomic landscape. We demonstrate single-cell co-assays of three small molecules together with histone modifications, chromatin accessibility or target proteins in human colorectal cancer (CRC) organoids. Integrated multimodal analysis reveals diverse drug interactions in the context of chromatin states within heterogeneous CRC organoids. We further reveal drug genomic binding dynamics and adaptive epigenome across cell types after small-molecule drug trea

SUBMITTER: Dong C 

PROVIDER: S-EPMC11399096 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature