Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

ABSTRACT: Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.

SUBMITTER: Konold PE 

PROVIDER: S-EPMC11399097 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature