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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

ABSTRACT: Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for direct observation of structural dynamics of many biochemical processes.

SUBMITTER: Konold PE 

PROVIDER: S-EPMC11399097 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature

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Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs.

Konold Patrick E PE   Monrroy Leonardo L   Bellisario Alfredo A   Filipe Diogo D   Adams Patrick P   Alvarez Roberto R   Bean Richard R   Bielecki Johan J   Bódizs Szabolcs S   Ducrocq Gabriel G   Grubmueller Helmut H   Kirian Richard A RA   Kloos Marco M   Koliyadu Jayanath C P JCP   Koua Faisal H M FHM   Larkiala Taru T   Letrun Romain R   Lindsten Fredrik F   Maihöfer Michael M   Martin Andrew V AV   Mészáros Petra P   Mutisya Jennifer J   Nimmrich Amke A   Okamoto Kenta K   Round Adam A   Sato Tokushi T   Valerio Joana J   Westphal Daniel D   Wollter August A   Yenupuri Tej Varma TV   You Tong T   Maia Filipe F   Westenhoff Sebastian S  

Nature methods 20240705 9

Detecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain. This time-resolved acquisition strategy is easy to implement and widely applicable for dire  ...[more]

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