Rapid, biochemical tagging of cellular activity history in vivo.

ABSTRACT: Intracellular calcium (Ca²⁺) is ubiquitous to cell signaling across biology. While existing fluorescent sensors and reporters can detect activated cells with elevated Ca²⁺ levels, these approaches require implants to deliver light to deep tissue, precluding their noninvasive use in freely behaving animals. Here we engineered an enzyme-catalyzed approach that rapidly and biochemically tags cells with elevated Ca²⁺ in vivo. Ca²⁺-activated split-TurboID (CaST) labels activated cells within 10 min with an exogenously delivered biotin molecule. The enzymatic signal increases with Ca²⁺ concentration and biotin labeling time, demonstrating that CaST is a time-gated integrator of total Ca²⁺ activity. Furthermore, the CaST readout ca

SUBMITTER: Zhang R 

PROVIDER: S-EPMC11399108 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature