MASTER-NAADP: a membrane permeable precursor of the Ca²⁺ mobilizing second messenger NAADP.

ABSTRACT: Upon stimulation of membrane receptors, nicotinic acid adenine dinucleotide phosphate (NAADP) is formed as second messenger within seconds and evokes Ca²⁺ signaling in many different cell types. Here, to directly stimulate NAADP signaling, MASTER-NAADP, a Membrane permeAble, STabilized, bio-rEversibly pRotected precursor of NAADP is synthesized and release of its active NAADP mimetic, benzoic acid C-nucleoside, 2'-phospho-3'F-adenosine-diphosphate, by esterase digestion is confirmed. In the presence of NAADP receptor HN1L/JPT2 (hematological and neurological expressed 1-like protein, HN1L, also known as Jupiter microtubule-associated homolog 2, JPT2), this active NAADP mimetic releases Ca²⁺ and increases the open probability of type 1 ryanodine receptor. When added to

SUBMITTER: Krukenberg S 

PROVIDER: S-EPMC11399135 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature