Comprehensive mapping of immune perturbations associated with aplastic anemia.

ABSTRACT:

Background

Aplastic anemia (AA) is an immune-mediated syndrome characterized by bone marrow failure. Therefore, comprehending the cellular profile and cell interactions in affected patients is crucial.

Methods

Human peripheral blood mononuclear cells (PBMCs) were collected from both healthy donors (HDs) and AA patients, and analyzed using multicolor flow cytometry. Utilizing the FlowSOM and t-SNE dimensionality reduction technique, we systematically explored and visualized the major immune cell alterations in AA. This analysis provided a foundation to further investigate the subtypes of cells exhibiting significant changes.

Results

Compared to HDs, peripheral blood from patients with AA exhibits a marked reduction in CD56^Dim natural killer (NK) cells, which

SUBMITTER: Wang H 

PROVIDER: S-EPMC11399290 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature