Simultaneous profiling of RNA isoforms and chromatin accessibility of single cells of human retinal organoids.

ABSTRACT: Single-cell multi-omics sequencing is a powerful approach to analyze complex mechanisms underlying neuronal development and regeneration. However, current methods lack the ability to simultaneously profile RNA alternative splicing and chromatin accessibility at the single-cell level. We develop a technique, single-cell RNA isoform and chromatin accessibility sequencing (scRICA-seq), which demonstrates higher sensitivity and cost-effectiveness compared to existing methods. scRICA-seq can profile both isoforms and chromatin accessibility for up to 10,000 single cells in a single run. Applying this method to human retinal organoids, we construct a multi-omic cell atlas and reveal associations between chromatin accessibility, isoform expression of fate-determining factors, and alternative spli

SUBMITTER: Zhang S 

PROVIDER: S-EPMC11399327 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature