Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.

ABSTRACT: Using ex vivo microscopy, virtual pathology can improve histological procedures by providing pathology images in near real-time without tissue destruction. Several emerging and promising approaches leverage fast-acting small-molecule fluorescent stains to replicate traditional pathology structural contrast, combined with rapid optical sectioning microscopes. However, several vital challenges must be addressed to translate virtual pathology into the clinical environment. One such challenge is selecting robust, reliable, and repeatable staining protocols that can be adopted across institutions. In this work, we addressed the effects of dye selection and staining protocol on image quality in rapid point-of-care imaging settings. For this purpose, we used structured illumination microscopy to

SUBMITTER: Bozic I 

PROVIDER: S-EPMC11399393 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature