Synthesis and screening of a library of Lewis^x deoxyfluoro-analogues reveals differential recognition by glycan-binding partners.

ABSTRACT: Glycan-mediated interactions play a crucial role in biology and medicine, influencing signalling, immune responses, and disease pathogenesis. However, the use of glycans in biosensing and diagnostics is limited by cross-reactivity, as certain glycan motifs can be recognised by multiple biologically distinct protein receptors. To address this specificity challenge, we report the enzymatic synthesis of a 150-member library of site-specifically fluorinated Lewis^x analogues ('glycofluoroforms') using naturally occurring enzymes and fluorinated monosaccharides. Subsequent incorporation of a subset of these glycans into nanoparticles or a microarray revealed a striking spectrum of distinct binding intensities across different proteins that recognise Lewis^x. Notably, we show

SUBMITTER: Hollingsworth K 

PROVIDER: S-EPMC11399408 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature